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    Keywords: CELLS ; EXPRESSION ; GROWTH ; BLOOD ; Germany ; RISK ; RNA ; RISK-FACTORS ; LESIONS ; PLASMA ; risk factors ; smoking ; LDL ; OVEREXPRESSION ; PERIPHERAL-BLOOD ; COX-2 ; LIPOPROTEINS ; N-ACETYL-CYSTEINE ; inflammation ; HYPERCHOLESTEROLEMIA ; SERUM ; ELISA ; free radicals ; MATRIX METALLOPROTEINASES ; ATHEROSCLEROTIC LESIONS ; GLUTAMATE LEVELS ; hyperlipoproteinemia ; NITRIC-OXIDE-SYNTHASE ; OXIDIZED LOW-DENSITY
    Abstract: Cyclooxygenase (COX)-2 is expressed in macrophages of arteriosclerotic lesions and promotes inflammation. We investigated whether COX-2 is already expressed in peripheral blood mononuclear cells (PBMCs) of subjects possessing risk-related factors, such as in smokers and hyperlipidemics. PBMCs were isolated from the venous blood of normolipidemic nonsmokers (NL-NSM; n = 15), normolipidemic smokers (NL-SM; n = 12), hyperlipidemic nonsmokers (HL-NSM; n = 10), and hyperlipidemic smokers (HL-SM; n = 10). RNA from PBMCs was used for RT-PCR. Plasma concentrations of oxidized low-density lipoproteins (oxLDL) were rneasured by ELISA, those of glutamate and cystine by HPLC. The results show that COX-2 expression in PBMCs was significantly increased in the groups with cardiovascular risk factors (NL-SM, HL-SM, HL-NSM) compared with NL-NSM. COX-2 expression in PBMCs was positively Correlated with concentrations of total serum cholesterol, oxLDL, glutamate, or cystine. We suggest that the elevated COX-2 expression indicates a priming of PBMCs as a response to a systemic pro-oxidative and proinflammatory shift in subjects with cardiovascular risk factors, which might also contribute to growth and instability of arteriosclerotic lesions. (C) 2004 Elsevier Inc. All rights reserved
    Type of Publication: Journal article published
    PubMed ID: 15607906
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