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    Keywords: RECEPTOR ; EXPRESSION ; GROWTH ; GROWTH-FACTOR ; proliferation ; Germany ; IN-VIVO ; NETWORKS ; SYSTEM ; GENE ; GENE-EXPRESSION ; HYBRIDIZATION ; transcription ; DIFFERENTIATION ; TISSUE ; COMPLEX ; COMPLEXES ; TRANSCRIPTION FACTOR ; AP-1 ; KERATINOCYTES ; SKIN ; C-JUN ; fibroblasts ; cytokines ; antibodies ; antibody ; MOUSE ; IN-SITU ; gene expression ; REPAIR ; SIGNALING PATHWAY ; HUMAN KERATINOCYTES ; Jun ; gene expression profiling ; expression profiling ; COLONY-STIMULATING FACTOR ; FACTOR GENE-EXPRESSION ; FUNCTIONAL EXPRESSION ; in situ hybridization ; keratinocyte ; regulation ; mesenchyme ; SDF-1 ; CHEMOKINE RECEPTORS ; INFLAMMATORY CYTOKINES ; CELL-DERIVED FACTOR ; CXCL12 ; GROWTH-ASSOCIATED MOLECULE ; HB-GAM ; LARGE INDUCTION ; pleiotrophin
    Abstract: In skin, fibroblasts of the connective tissue play a decisive role in epidermal homeostasis and repair by contributing to the regulation of keratinocyte proliferation and differentiation. The AP-1 transcription factor subunit JUN plays a crucial role in this mesenchymal-epithelial interplay by regulating the expression of two critical paracrine-acting cytokines, keratinocyte growth factor (KGF) and granulocyte-macrophage colony-stimulating factor (GMCSF). We have performed gene expression profiling of wildtype and Jun(-/-) mouse embryonic fibroblasts to identify additional players involved in this complex network, and have found pleiotrophin (PTN) and the stromal cell-derived factor 1 (SDF-1) as novel JUN-regulated factors. Both cytokines are expressed by dermal fibroblasts in vivo, as shown by semi-quantitative RT-PCR and in situ hybridization on murine skin sections. Using a heterologous feeder layer co-culture system, we demonstrated that PTN and SDF-1 exert a mitogenic effect on primary human keratinocytes. Moreover, SDF-1-induced keratinocyte proliferation could be specifically inhibited by neutralizing antibodies against SDF-1 or its receptor, CXCR4. Consistent with its role in promoting keratinocyte growth, PTN was upregulated during cutaneous wound healing in vivo. Interestingly, co-cultivation with keratinocytes stimulated PTN expression but repressed SDF-1 production in fibroblasts, demonstrating the complexity of the paracrine regulatory cytokine networks that control skin homeostasis and regeneration
    Type of Publication: Journal article published
    PubMed ID: 15840658
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