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    Keywords: RECEPTOR ; EXPRESSION ; CELL ; Germany ; GENERATION ; SYSTEM ; GENE-EXPRESSION ; RNA ; EFFICIENCY ; MICE ; TRANSCRIPTION FACTOR ; BIOLOGY ; MOLECULAR-BIOLOGY ; ACIDS ; TRANSGENIC MICE ; NUCLEAR-FACTOR ; DESIGN ; VECTOR ; LINE ; DELIVERY ; MAMMALIAN-CELLS ; STEM-CELLS ; NETHERLANDS ; molecular biology ; molecular ; RE ; INTERFERENCE ; RNA INTERFERENCE ; GAMMA ; LENTIVIRAL VECTOR ; methods ; NUCLEAR ; function ; TRANSMISSION ; microbiology ; lentiviral vectors ; MicroRNAs ; mosaicism ; biotechnology ; INTERFERING RNAS ; SIRNAS ; Transgenesis ; TRANSGENIC RATS
    Abstract: We have used a lentiviral delivery system (LentiLox3.7) to generate transgenic mice harbouring RNA interference (RNAi) against the hepatocyte nuclear factor 4 gamma (HNF4(gamma)). HNF4(gamma) is a nuclear receptor with unknown function. Our analyses performed on founder (F-0) and first generation (F-1) mice revealed mosaicism in F-0 founders and a low efficiency of transgenesis (6%) in F-1 mice. These data, together with the observation of multiple silenced transgenes, do not favour the use of LentiLox3.7 lentivirus for transgenesis. Despite the low efficiency of transgenesis, we achieved a tissue-dependent knockdown of HNF4(gamma) expression in some mice
    Type of Publication: Journal article published
    PubMed ID: 17682835
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