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    Keywords: APOPTOSIS ; CANCER ; CELLS ; GROWTH ; IRRADIATION ; proliferation ; tumor ; TUMOR-CELLS ; MODEL ; CARCINOGENESIS ; KERATINOCYTES ; MOUSE ; EQUIVALENT ; PROGRESSION ; MUTATION ; MUTATIONS ; NEOPLASTIC PROGRESSION ; intraepithelial neoplasia ; organotypic culture ; HA-RAS ; HACAT-RAS ; HUMAN SKIN ; MALIGNANT KERATINOCYTES ; RECONSTRUCTED IN-VITRO ; SUNLIGHT ; UVB irradiation
    Abstract: Ultraviolet B irradiation is thought to enable skin cancer progression as clones of genetically damaged keratinocytes escape apoptosis and expand at the expense of adjacent normal cells. Mechanisms through which potentially malignant cells in human skin undergo clonal expansion, however, are not well understood. The goal of this study was to characterize the role of ultraviolet B irradiation on the intraepithelial expansion of early stage human tumor cells in organotypic skin cultures. To accomplish this, we have studied the effect of ultraviolet B irradiation on organotypic cultures that were fabricated by mixing normal human keratinocytes with beta-galactosidase- marked, intraepithelial tumor cells (HaCaT-ras , clone II-4), which bear mutations in both p53 alleles and harbor an activated H-ras oncogene. We found that when organotypic mixtures were exposed to an ultraviolet B dose of 50 mJ per cm(2) , intraepithelial tumor cells underwent a significant degree of proliferative expansion compared to nonirradiated cultures. To understand this response, organotypic cultures of nor-mal keratinocytes were exposed to ultraviolet B and showed a dose-dependent increase in numbers of sunburn cells and TUNEL-positive cells although their proliferation was suppressed. In contrast, neither the apoptotic nor the proliferative response of II-4 cells was altered by ultraviolet B in organotypic cultures. The differential response of these cell types suggested that II-4 cells were resistant to ultraviolet-B-induced alterations, which allowed these intraepithelial tumor cells to gain a selective growth and survival advantage relative to neighboring normal cells. These findings demonstrate that ultraviolet B exposure can induce the intraepithelial expansion of apoptosis-resistant, p53-mutant, and ras -activated keratinocytes, suggesting that this agent can act to promote the early stages of epithelial carcinogenesis
    Type of Publication: Journal article published
    PubMed ID: 12839581
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