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    ISSN: 1432-072X
    Keywords: Rhizobium isolates ; Uptake hydrogenase ; Native ecosystem ; Cow pea miscellany hosts ; Hup+ and Hup- Rhizobium strains ; Acetylene reduction activity (ARA) ; Competence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Rhizobium strains nodulating summer legumes cow pea [Vigna unguiculata (L.)], green gram [V. radiata (L.) (Wilczek)], black gram [V. mungo (L.) (Hepper)] and cluster bean [Cyamopsis tetragonoloba (L.) (Taub)] and a winter legume chick pea [Cicer arietinum (L.)] were surveyed in the Northern Plains of India and screened for hydrogenase activity to determine distribution of Hup character in the native ecosystem. It was observed that 56% of the Rhizobium strains of summer legumes were Hup+ whereas that of the winter legume, chick pea, were all Hup-. Ex planta acetylene reduction activity was observed in most of the Hup+ but not in the Hup- strains of any of the host species. In summer legume, mixed inoculation of Hup+ and Hup- strains, under sterilized as well as unsterilized soil conditions, showed that the host species were predominantly nodulated with Hup+ strain.
    Type of Medium: Electronic Resource
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