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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Current genetics 21 (1992), S. 83-84 
    ISSN: 1432-0983
    Keywords: Yeast ; Transformation ; Thio compound ; Stationary phase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A fast yeast-transformation technique has been developed by adding thio compounds to alkali-ion based protocols and incubating at 45°C. This procedure is especially recommended for cells from stationary phase at a density up to 2.5×108 cells/ml. It involves only one step for the preparation and transformation of competent cells within 30 min. The yield was more than 104 transformants/μg plasmid DNA. This protocol is easy to scale up for many DNA samples and is also applicable for yeast cells from different types of storages.
    Type of Medium: Electronic Resource
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