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  • 1
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung In der Harderschen Drüse von Kaninchen wurden mit der Tetrazoliumtechnik die Enzyme NADH-Tetrazoliumreduktase (NADH-T-Red), NADPH-Tetrazoliumreduktase (NADPH-T-Red), NAD-spezifische Isocitrat-Dehydrogenase (NAD-IDH), Succinat-Dehydrogenase (SDH), NAD-spezifische Malat-Dehydrogenase (NAD-MDH), Laktat-Dehydrogenase (LDH), Glucose-6-phosphat-Dehydrogenase (G-6-PDH),α-Glycerophosphat-Dehydrogenase (GPDH) undβ-Hydroxybuttersäure-Dehydrogenase (HBDH) histochemisch nachgewiesen und ihr Verteilungsbild in den beiden Drüsenlappen studiert. Darüber hinaus wurden histochemische Reaktionen auf folgende Enzyme durchgeführt: Cytochromoxydase (nachBurston), Leucinaminopeptidase (nachNachlas,Crawford undSeligman), alkalische Phosphatase (nachGomori), ATPase (nachPadykula undHerman). Fette wurden mit Scharlachrot und Sudan Schwarz B gefärbt. Die histochemisch faßbare Aktivitätsverteilung der Enzyme geht aus der Tabelle 1 hervor. Die Befunde werden diskutiert.
    Notes: Summary A study was conducted to investigate the distribution pattern of various enzymes in both lobes of the Harder gland in rabbits by means of the tetrazolium technique [NADH-tetrazoliumreductase (NADH-T-Red), NADPH-tetrazolium-reductase (NADPH-T-Red), NAD-specific isocitrate-dehydrogenase (NAD-IDH), succinate-dehydrogenase (SDH), NAD-specific malate-dehydrogenase (NAD-MDH), lactate-dehydrogenase (LDH), glucose-6-phosphate-dehydrogenase (G-6-PDH),α-glycerophosphate-dehydrogenase (GPDH), andβ-hydroxy-butyrate-dehydrogenase (HBDH)]. In addition histochemical tests for the determination of cytochrome-oxidase (Burston), leucine-amino-peptidase (Nachlas,Crawford andSeligman), alkaline phosphatase (Gomori), and ATP-ase (Padykula andHerman) were carried out. Fets were stained with Scharlach-red and Sudan black B. The results of the histochemically determinable enzyme activities are listed in table 1. The findings are discussed.
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