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  • 1
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Neurokinin-1 (NK-1)/substance P (SP) receptors were solubilized using 10 mM 3-[(cholamidopropyl)-dimethylammonio]-1-propanesulfate from porcine striatal membranes (solubilization yield, 80%). In solubilized preparations, [3H]SP apparently bound to a single class of high-affinity sites (KD= 0.82 ± 0.13 nM) as in membrane ho-mogenates. The ligand selectivity pattern observed in both membrane and solubilized receptor preparations indicated that [Sar9, Met(O2)11]SP = SP ± senktide = [Nle10]neurokinin A4–10.This suggests the selective labeling of the NK-1 receptor class in both assays. Solubilized receptors were retained on agarose-coupled lectins that bind N-acetylglucosamine-galactose and β-galactose (Ricinus communis I and Ricinus communis II), mannose (concanavalin A and lentil), and N- acetylglucosamine (wheat germ agglutinin) but not on lectins binding fucose (Lotus A) and N-acetylgalactosamine (Dolichos biflorus A). Thus, it appears that porcine brain NK-1/SP receptors are enriched with various carbohydrate moieties, β-galactose and N-acetylglucosamine-galactose residues being especially abundant. This situation is rather different from that in various other members of the rhodopsin seven-trans-membrane receptor superfamily.
    Type of Medium: Electronic Resource
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