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  • 1
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: Rat brain synaptic vesicles exhibit ATP-dependent uptake of γ-[3H]amino-n-butyric acid ([3H]GABA) and l-[3H]glutamate. After hypotonic shock, the highest specific activities of uptake of both l-glutamate and GABA were recovered in the 0.4 M fraction of a sucrose gradient. The uptakes of l-glutamate and GABA were inhibited by similar, but not identical, concentrations of the mitochondrial uncoupler carbonyl cyanide m-chlorophenylhydrazone and the ionophores nigericin and gramicidin, but they were not inhibited by the K+ carrier valinomycin. N, N′-Dicyclohexyl-carbodiimide and N-ethylmaleimide, Mg2+-ATPase inhibitors, inhibited the GABA and L-glutamate uptakes similarly. Low concentrations of CI− stimulated the vesicular uptake of l-glutamate but not that of GABA. The uptakes of both l-glutamate and GABA were inhibited by high concentrations of CI−. These results indicate that the vesicular GABA and l-glutamate uptakes are driven by an electrochemical proton gradient generated by a similar Mg2+-ATPase. The vesicular uptake mechanisms are discussed in relation to other vesicle uptake systems.
    Type of Medium: Electronic Resource
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